Cell Hashing from Satija Lab

Cell Hashing from Satija Lab

Single-cell sequencing, one of the cornerstone nascent technologies for the HuBMAP, is driving a new wave of biological insight. Single-cell sequencing can drive deeper understanding of our immune system or cancer progression, and even discovery new cell types. Some of the technological challenges to the emerging technologies supporting single cell sequencing include cost, batch effects, and technical artifacts. In this open access paper, a team of scientists at the New York Genome Center (NYGC) describe a new method called “Cell Hashing” to help address these challenges. The method uses DNA-barcoded antibodies to place labels (‘hashtags’) on individual cells, enabling multiple samples to be mixed together and profiled in a single experiment. The study was jointly led by the Satija Lab, which leads a HuBMAP Mapping Center, and the Technology Innovation lab at NYGC.